DSM No.:
3421 dsm-3421 dsm3421 dsm 3421 pUC9
History:
<- I. Oberbäumer <- J. Messing
Remarks:
Expression vector; cloning vector with multiple restriction enzyme linker; derived from pBR322 and M13mp9-vector; pUC9 allows direct selection of cloned DNA because of α complementation; allows using the same primer as for the M13 system; pUC9 allows to clone double-digested restriction fragments separately in both orientation with respect to the lac promoter; replicon: pMB1; not self-transmissible.
Distributed in:
Escherichia coli K12 JM83
Risk group:
1 (classification according to German
TRBA)
Restrictions:
German law regulating genetic engineering / Gentechnikgesetz),
Category C
Country:
country of origin unknown
Date of sampling:
before 16.03.1990
Nagoya Protocol Restrictions:
There are NO known Nagoya protocol restrictions for this strain.
