DSM No.:
4862 dsm-4862 dsm4862 dsm 4862 pNF2
Other collection no.
or WDCM no.:
ATCC 37187
History:
<- E.C. Friedberg
Marker:
Apr and Kmr in E. coli, G418r and Ura+ in yeast
Remarks:
Cloning vector with 2μ plasmid replicon for Saccharomyces cerevisiae and Escherichia coli. pNF2 is a derivative of the multicopy yeast cloning vector YEp24 and contains a single BamHI cloning site flanked immediately on each side by SalI sites. DNA fragments cloned into the BamHI site that do not regenerate this restriction site can be excised by digestion with SalI enzyme. The Kmr gene derived from Tn903 confers resistance in yeast to the antibiotic G418.
Distributed in:
Escherichia coli K12 HB101
Risk group:
1 (classification according to German
TRBA)
Restrictions:
German law regulating genetic engineering / Gentechnikgesetz),
Category C
Country:
country of origin unknown
Date of sampling:
before 16.03.1990
Nagoya Protocol Restrictions:
There are NO known Nagoya protocol restrictions for this strain.
