DSM No.:
4863 dsm-4863 dsm4863 dsm 4863 pNF3
Other collection no.
or WDCM no.:
ATCC 37188
History:
<- E.C. Friedberg
Marker:
Apr and Kmr in E. coli, G418r and Ura+ in yeast
Remarks:
pNF2 (DSM 4862) was converted into the integrating vector pNF3, a vector without a replicon for yeast. Since pNF3 carries the URA3 gene and the kamr gene from Tn903 as selection markers for Saccharomyces cerevisiae, DNA sequences containing a replicon for Saccharomyces cerevisiae can be selected. pNF3 has a unique BamHI cloning site flanked by SalI sites on both ends.
Distributed in:
Escherichia coli K12 HB101
Risk group:
1 (classification according to German
TRBA)
Restrictions:
German law regulating genetic engineering / Gentechnikgesetz),
Category C
Country:
country of origin unknown
Date of sampling:
before 16.03.1990
Nagoya Protocol Restrictions:
There are NO known Nagoya protocol restrictions for this strain.
