The transposon Tn1725 (8.9 kb) was inserted in vivo into the conjugative Kmr ts plasmid Rts1 (Rts1::Tn1725); Tn1725 itself is a Cmr variant of Tn1722 obtained by ligation of a 3.3 kb HindIII fragment of R plasmid Sa that contains the cat gene into the single HindIII site of Tn1722; useful for mutagenesis in Escherichia coli; the large size of Rts1 (217 kb) prevents cotransformation of any Rts1::Tn replicons.
German law regulating genetic engineering / Gentechnikgesetz), Category C Material transfer agreement, Category DCommercial use and transfer to third parties is completely excluded for this strain.
Country:
country of origin unknown
Date of sampling:
before 16.03.1990
Nagoya Protocol Restrictions:
There are NO known Nagoya protocol restrictions for this strain.
