The transposon Tn1731 (7.8 kb) was inserted in vivo into the conjugative Kmr ts plasmid Rts1 (Rts1::Tn1731); Tn1731 itself is a Tcr variant of Tn1722, an in vitro recombinant of Tn1722 and a 2.4 kb EcoRI fragment carrying the tet genes of Tn1721; the EcoRI fragment was derived from plasmid pJOE452; useful for mutagenesis in Escherichia coli; the large size of Rts1 (217 kb) prevents cotransformation of any Rts1::Tn replicons.
German law regulating genetic engineering / Gentechnikgesetz), Category C Material transfer agreement, Category DCommercial use and transfer to third parties is completely excluded for this strain.
Country:
country of origin unknown
Date of sampling:
before 16.03.1990
Nagoya Protocol Restrictions:
There are NO known Nagoya protocol restrictions for this strain.
