DSM No.:
4963 dsm-4963 dsm4963 dsm 4963 pRU663
Marker:
Apr, Cmr, incP, Tcr, tra+, trfAts
Remarks:
The transposon Tn1725 (8.9 kb) was inserted in vivo into the ts conjugative broad-host-range R plasmid pME305 (Apr, Tcr) (pME305::Tn1725); Tn1725 itself is a Cmr variant of Tn1722 obtained by ligation of a 3.3 kb HindIII fragment of R plasmid Sa that contains the cat gene into the single HindIII site of Tn1722; useful for mutagenesis in various Gram-negative bacteria.
Distributed in:
Escherichia coli K12 JM108
Risk group:
1 (classification according to German
TRBA)
Restrictions:
German law regulating genetic engineering / Gentechnikgesetz),
Category CMaterial transfer agreement,
Category DCommercial use and transfer to third parties is completely excluded for this strain.
Country:
country of origin unknown
Date of sampling:
before 16.03.1990
Nagoya Protocol Restrictions:
There are NO known Nagoya protocol restrictions for this strain.
