pRU672
DSM 4965
PLASMIDS
Name:
pRU672
DSM No.:
4965 dsm-4965 dsm4965 dsm 4965 pRU672
History:
<- R. Schmitt
Marker:
Apr, incP, Kmr, Smr (not expressed in E. coli host), Tcr, tra+, trfAts
Remarks:
The transposon Tn1733 (8.9 kb) was inserted in vivo into the ts conjugative broad-host-range R plasmid pME305 (Apr, Tcr) (pME305::Tn1733); Tn1733 itself is a Kmr (Smr) variant of Tn1722, obtained by ligation of a 3.3 kb HindIII fragment that comprises the central region of Tn5 into the single HindIII site of Tn1722; the cloned fragment encodes Kmr, ble and str (str is cryptic in Escherichia coli, but is expressed in different nonenteric bacteria); useful for mutagenesis in various Gram-negative bacteria.
Distributed in:
Escherichia coli K12 MM294
Cultivation conditions:
Medium 381, 30°C
Risk group:
1 (classification according to German TRBA)
Restrictions:
German law regulating genetic engineering / Gentechnikgesetz), Category C
Material transfer agreement, Category DCommercial use and transfer to third parties is completely excluded for this strain.
Material transfer agreement, Category DCommercial use and transfer to third parties is completely excluded for this strain.
Country:
country of origin unknown
Date of sampling:
before 16.03.1990
Nagoya Protocol Restrictions:
There are NO known Nagoya protocol restrictions for this strain.
Supplied as:
Delivery form | Organization type | ||||
Profit | Non-Profit | ||||
Freeze Dried | 100,- € | 100,- € | |||
Active culture on request | 240,- € | 240,- € |
Price category 1 strain.
Please see price list for freight and handling charges