Leibniz Institute DSMZ: Details

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PLASMIDS

Name:

pKC7

DSM No.:

5163 dsm-5163 dsm5163 dsm 5163 pKC7

Other collection no.
or WDCM no.:

ATCC 37084, NCIB 12389

History:

<- G. Auling <- M. Hahn

Mol. weight:

5.6 kb

Marker:

Ap^r, Km^r

Remarks:

Construction: pBR322, lambda, Tn5; cloning vector; replicon: pMB1; promoter: lambdaPL; for overproduction of gene product; permits cloning of DNA segments cleaved with ten different restriction endonucleases; replicon ColE1, relaxed; single restriction sites: SmaI, XhoI, BstEII, BamHI, PvuI, EcoRI, HindIII, BglII, BclI, SalI; insertion of foreign DNA into the BglII site inactivates the Km^r gene.

Distributed in:

Escherichia coli N100

Cultivation conditions:

Medium 381, 37°C

Risk group:

1 (classification according to German TRBA)

Restrictions:

German law regulating genetic engineering / Gentechnikgesetz), Category C

Country:

country of origin unknown

Date of sampling:

before 16.03.1990