DSM No.:
6185 dsm-6185 dsm6185 dsm 6185 YEp24
Other collection no.
or WDCM no.:
ATCC 37051, CCRC 41112
History:
<- CCRC <- New England Bio-Labs <- ATCC
Remarks:
Yeast episomal plasmid with numerous unique restriction sites. Cloning vehicle for introduction of constructs into S. cerevisiae at a high copy number. Can replicate in both E. coli (due to pBR322 replication origin) and S. cerevisiae (due to the replication determinant of the yeast 2 micron circle plasmid). Apr is the selectable marker in E. coli, the URA3 gene of the yeast strain +D4 usable as selective marker in yeast. The promoter of the Tcr gene of pBR322 is separated by the URA3 sequence.
Distributed in:
Escherichia coli K12 HB101
Risk group:
1 (classification according to German
TRBA)
Restrictions:
German law regulating genetic engineering / Gentechnikgesetz),
Category C
Country:
country of origin unknown
Date of sampling:
before 02.08.1990
Nagoya Protocol Restrictions:
There are NO known Nagoya protocol restrictions for this strain.
