DSM No.:
6699 dsm-6699 dsm6699 dsm 6699 pUTK21
History:
<- G.S. Sayler, Univ. Tennessee
Remarks:
Bioluminescent reporter plasmid for naphthalene catabolism, developed by insertion of Tn4431 of the lux gene cassette from Vibrio fischeri into a naphthalene catabolic plasmid in P. fluorescens. Insertion site of the lux transposon was the nahG gene encoding for salicylate hydroxylase. Luciferase-mediated light production from P. fluorescens strains harbouring this plasmid is induced on exposure to naphthalene or the regulatory inducer metabolite salicylate. In continuous culture, rapid light production occurs after 15 minutes. This strain does not mineralize naphthalene completely and accumulates salicylate, due to insertion in the lower pathway operon. The lux transposon is located on a 1.6 kb PstI fragment adjacent to the nahH gene.
Distributed in:
Pseudomonas fluorescens 5RL
Risk group:
1 (classification according to German
TRBA)
Restrictions:
German law regulating genetic engineering / Gentechnikgesetz),
Category CMaterial transfer agreement,
Category DCommercial use and transfer to third parties is completely excluded for this strain.
Country:
country of origin unknown
Date of sampling:
before 06.03.1995
Nagoya Protocol Restrictions:
There are NO known Nagoya protocol restrictions for this strain.
