DSM No.:
8820 dsm-8820 dsm8820 dsm 8820 pGEc228
History:
<- J. van Beilen, ETH Zürich
Remarks:
The alkST sequence has been cloned into the vector pJRD158. The alkS cistron encodes a 99-kDa protein responsible for the activation of the expression of the alkBAC operon, the alkT cistron encodes a 48-kDa protein, required for reconstitution of the Alk phenotype (component of the alkane hydroxylase complex). This subclone carries a 4.9 kb SalI fragment and a functional alkR locus. The plasmid is only selectable with the Ampicillin marker. The cloned alk genes are covered by U.S. patent 5,173,428. For general information see pGEc41.
Distributed in:
Escherichia coli K12 GEc137
Risk group:
1 (classification according to German
TRBA)
Restrictions:
German law regulating genetic engineering / Gentechnikgesetz),
Category CMaterial transfer agreement,
Category DCommercial use and transfer to third parties is completely excluded for this strain.
Country:
country of origin unknown
Date of sampling:
before 07.07.1995
Nagoya Protocol Restrictions:
There are NO known Nagoya protocol restrictions for this strain.
