Species:
human (Homo sapiens)
Cell type:
acute monocytic leukemia
Origin:
established from the peripheral blood of a 64-year-old man with relapsed acute monocytic leukemia (AML FAB M5) in 1985 following myeloid metaplasia; carries t(9;11)(p22;q23) leading to KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) fusion gene; more information on this cell line on the following website: "www.monocyte.eu"; sister cell line of MONO-MAC-1 (DSM ACC 252); cells are used for the detection of pyrogens. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Depositor-specific restrictions:
Commercial entities need a permit from the original investigator (please contact DSMZ: mutz@dsmz.de). Transfer to third parties needs to be negotiated with the depositor.
Morphology:
single, round/multiformed cells or small clusters of cells in suspension, sometimes loosely adherent; 1-5% giant cells;
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Medium:
90% RPMI 1640 + 10% h.i. FBS + 2 mM L-glutamine + non-essential amino acids + 1 mM sodium pyruvate + 10 µg/ml human insulin
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at 0.3-1.0 x 106 cells/ml; split ratio 1:3 to 1:5; avoid lipopolysaccharide contamination which will result in cell clumping, maturation and retarded growth; cells are best started
in 12- or 24-well plates
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-60 hours
Harvest:
maximal density at ca. 1.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD4 -, CD13 +, CD14 +, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +; note that CD14 expression depends on cultivation conditions; CD14 positivity cannot be guaranteed;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypotetraploid karyotype with near-diploid (8%) and polyploid (17%) sidelines - 84-90<4n>XX/XXX, -Y, +6, +7, -12, -13, -13, -16, -16, +2mar, t(9;11)(p22;q23)x2, add(10)(p11)x2, add(12)(q21), del(13)(q13q14)der(13)t(13;14)(p11;q12)x2, der(17)t(13;17)(q21;p11)x2 - first cell line shown to carry t(9;11) characteristic of AML M5
Molec. Genetics:
expression of fusion gene KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
