Species:
human (Homo sapiens)
Cell type:
chronic myeloid leukemia in blast crisis
Origin:
established from the bone marrow of a 5-year-old girl with Philadelphia chromosome positive chronic myeloid leukemia (CML) in second relapse in 1980 (after bone marrow transplantation day +28); sister cell line of EM-3 (DSMZ ACC 134). Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.3-0.5 x 106 cells/ml; maintain at 0.2-1.0 x 106 cells/ml; split ratio of 1:2 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35-40 h
Harvest:
maximum density at about 1.0-1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, HLA-DR -;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypertriploid karyotype with hypotetraploid sideline - 74(70-86)<3n>X, -X, -X, +3, +4, +6, +6, +6, +8, -9, +11, -14, -14, +15, +17, -19, +21, +22, +mar - der(5)t(5;?)(q13-15;?), der(9)t(9;22)(q34;q11), i(17q)x2 - in some cells up to three copies of der(9) are present - the karyotype overlaps that of EM-3 (DSM ACC 134) and corresponds with those reported for both EM-2 and EM-3
Molec. Genetics:
expression of fusion gene BCR-ABL1 e14-a2 (b3-a2) confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
