LNCAP
ACC 256
Cell line:
LNCAP
DSMZ no.:
ACC 256
Species:
human (Homo sapiens)
Cell type:
prostate carcinoma
Origin:
established from the left supraclavicular lymph node metastasis from a 50-year-old man with prostate carcinoma in 1977; cells were described in the literature to be androgen-sensitive
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/25 cm2; split semi-confluent culture 1:4 once a week using trypsin/EDTA (occasionally cells can be detached by tapping); after thawing and trypsinization cells may need 1-2 days to become adherent again; due to the strong cell aggregation, it might be difficult to determine an exact cell titer
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 60 hours
Harvest:
cell harvest of about 10-15 x 106 cells/80 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypotetraploid karyotype with 6% polyploidy - 88(84-93)<4n>XXYY, -2, -4, -6, -6, -13, -13, +2mar, t(1;15)(p22.3;q22.3)x2, del(2)(p16p23), del(10)(q24)x2, der(16)t(6;16)(p22.1;q22)x2 - del(10)(q24) is recurrent in prostate carcinoma - corresponds to published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list