Species:
human (Homo sapiens)
Origin:
established from the biopsy from a fibrosarcoma of a 35-year-old man in 1972; patient did not receive any chemo- or radiotherapy
Permissions and restrictions:
Medium:
90% DMEM + 10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture up to 1:10 every 3-5 days using trypsin/EDTA
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 30 hours
Harvest:
cell harvest of ca. 25 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-diploid karyotype with 24% polyploidy - 46<2n>XY, der(5)t(5;5)(p15;q32), der(11)t(10;11)(q24;q11) - sideline with -13, del(1)(p32), del(1)(q24) - 5p15 is a putative recurrent breakpoint in fibrosarcoma
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
