Which viruses are detected in human and primate cell lines?
We have established PCR assays for the detection of the human pathogenic viruses including Hepatitis B viruses (HBV), Hepatitis C viruses (HCV), Human immunodeficiency virus type 1 (HIV1), Human immunodeficiency virus type 2 (HIV2), and Human T-cell leukemia virus types I and II (HTLV-I/-II). To determine the transformation of B cells by Epstein Barr virus, we also established an assay for the detection of EBV, which is categorized as risk group 2.
Additionally, we are able to detect Human herpes virus type 8 (HHV-8) and Transfusion transmitted virus (TTV).
How much material should I send?
For viable cells, about 5x10E6 cells are sufficient for the nucleic acid extractions. For HBV, about 10 ml supernatant should be sent.
For DNA/RNA preparations, the nucleic acids from 2-5x10E6 cells should suffice.
What does the report look like?
The report consists of a description of the method, the result of the analysis, and a photo of the ethidium bromide stained gel.