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Plant Cell LinesPlant Cell Lines

Pictures of Plant Cell Lines Department

Maintenance of cell cultures

Plant cell cultures are routinely maintained as calli by continuous sub-culturing at DSMZ. They are stored in cabinets with several trays. Each of the trays (60 x 60 cm) carries cell cultures of 100 different plant species.

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Callus cultures

When plant tissue, like leaf, stem or root material is surface sterilized and under strictly sterile conditions grown on media containing a specific composition of plant growth regulators, single cells develop an undifferentiated tumor like growth. Such a plant cell tumor -called callus - often retains metabolic features of the intact plant like secondary metabolite production.

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Callus from grape

Sometimes plant cell cultures show a strong production of secondary plant metabolites known from specialized tissues of the intact plant. As an example the picture shows a callus culture of grapes (Vitis vinifera L.).

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"Hairy root" culture

An infection of plants with the soil bacterium Rhizobium rhizogenes (Riker et al. 1930) Young et al. 2001 leads to a genetic transformation of plant cells. Such cells form roots which can be propagated on artificial media in petri dishes or suspended in liquid media. These „hairy roots“ can be used to investigate the metabolism of plant roots or to produce plant root metabolites.

©DSMZ

Cell Clusters 1

The picture shows a cluster of single plant cells in a suspension culture under the microscope at a magnification of 400x. After cell division cells of a suspension culture normally stick together and form such small cell clusters.

©DSMZ

Cell Clusters 2

The picture shows a cluster of single plant cells in a suspension culture under the microscope at a magnification of 400x. After cell division cells of a suspension culture normally stick together and form such small cell clusters.

©DSMZ