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Insect VectorsInsekten-Vektoren

Identification of genes and proteins involved in circular transmission of begomoviruses in whiteflies

Focus of this GIF-funded project is the characterization of molecular factors involved in circulative transmission of begomoviruses (Fam. Geminiviridae) by their vector whitefly Bemisia tabaci biotype B.

Proteome studies with efficiently and poorly transmitting whitefly populations aim at identification of insect proteins specifically interacting with virus coat proteins.
Molecular studies intend to resolve the role of bacterial endosymbiont colonization for virus translocation.
In situ immunological and hybridization studies explore the organ barriers for virus translocation. Results show that both the decending midgut and the salivary glands are barriers of virus translocation.

 

TYLCV, WmCSV, VOPBA, proteomics, co-localization

 

Virus overlay protein binding assays of whitefly proteins with purified Watermelon chlorotic stunt virus particles reveal several whitefly proteins (arrows) with specific affinity to virus coat protein. Proteins are identified by Electrospray Ionisation Mass Spectrometry and further studied to in situ for functional confirmation in vivo.

 

PI: Dr. Monika Götz

 

This project was supported by the German-Israeli Foundation for Scientific Research and Development (GIF) grant.


Cooperation partner:
Hanokh Czosnek, Hebrew University of Jerusalem, Rehovot, Israel
Murad Ghanim, Volcani Center, Bet Dagan, Israel

Referenz:
Götz M, Popovski S, Kollenberg M, Gorovits R, Brown JK, Cicero JM, Czosnek H, Winter S, Ghanim M. 2012. Implication of Bemisia tabaci heat shock protein 70 in Begomovirus-whitefly interactions. J. Virol. 86(24): 13241-52.