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Introduction of virus resistance in plants by expression of virus derived genes, artificial microRNAs and synthetic gene constructs presents an option when natural resistance is not available


Virus diversity analysis defines target viruses.




In transient expression assays using GFP reoporter genes putative viral sequences are defined to designe effective target constructs.

                                                               Using Nicotiana benthamiana as model plant to study plant response to virus infection extend and spectrum of resistance is tested to provide specifically virus resistance designed to target virus species and/or strains.


Identification and assessment of virus resistance in crops is a major effort, done in collaboration with breeders and laboratories of the international agricultural research institutes around the world.
Virus infection studies identified resistant cassava genotypes through inoculation screening with defined reference viruses.



Various responses, from immunity and recovery resistance to high susceptibility were obtained in cassava cultivars.


Using an agilent micro array, developed in collaboration with international partners comprising 11000 unigens from EST libraries of cassava, genes differentialy regulated during various virus conditions initiated under standard conditions were identified.  PI: Dr. Stephan Winter