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21. How do I know the lot no. of an ampoule and what does it mean?  
The preservation date on the inner vial is the lot identification. Viability and authenticity of each strain is routinely tested at the DSMZ so that ampoules that are several years old can be used…  
22. Which batch number/culture transfers in the sense of lab standards are DSMZ ampoules or actively growing cultures?  
Our freeze-dried microorganisms in glass ampoules are batch no. zero, actively growing cultures are batch no. one.  
23. What does the ampoule pellet consist of?  
A freshly cultivated cell suspension of the microorganism is either dropped onto a pre-dried protectant/carrier or the culture suspension is mixed with a liquid protectant/carrier. Depending on the…  
24. How many cells does an ampoule contain?  
The cell numbers present in a pellet differ due to strain-specific preservation conditions. Each lot is routinely checked for strain viability so that the DSMZ guarantees that a sufficient number of…  
25. Why do some ampoules contain blue, others red indicator?  
“Silica Gel Orange” replaces silica gel (with a blue indicator) in order to minimise the use of hazardous chemicals. Both indicators change colour in the presence of humidity (indicating damaged…  
26. Why do some ampoules contain a black pellet?  
Several strains of fastidious microorganisms, especially strictly anaerobic bacteria, are suspended prior to lyophilization in a protectant mixture containing ferrous sulfide or charcoal, which gives…  
27. How to reactivate fastidious bacteria from ampoules?  
For many fastidious organisms a higher inoculum is needed to reactivate them. Add 0.5 ml of adequate liquid medium (given for each strain in its specific strain entry) to the pellet and dissolve the…  
28. If no liquid medium is recommended in the specific strain entry, which solution do I use to solubilize the freeze-dried pellet (reactivation) or to cultivate the strains in liquid medium?  
Generally, use the recommended medium without the addition of agar. For fastidious microorganisms which grow only on solid media (e.g. M 693; M 429; M 585) use complex liquid media like M 1; M 545 or…  
29. What is the expiry date of freeze-dried cultures in unopened ampoules?  
The DSMZ does not set an expiry date on freeze-dried cultures. Our Terms and Conditions state that all cultures are meant for immediate use only, indicating a warranty period of four weeks. Most…  
30. Can I receive certificates for the strains I ordered stating that they are free of components of animal origin or components bearing TSE / BSE (Transmissible Spongiforme Encephalopathy / Bovine Spongiforme Encephalopathy)?  
No, the DSMZ does not certify such data. The providers of media and other products may be in a position to certify this information. The DSMZ Department of Microorganisms only uses high-quality…  
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