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Analysis of whole-cell sugarsAnalysis of whole-cell sugars

Analysis of whole-cell sugars

Either a culture of the strain or 15 mg lyophilized cells are needed.

The cells are hydrolyzed (1N H2SO4, 100 °C, 2 hours) and the sugars are analyzed by TLC on cellulose plates according to Staneck & Roberts (1974).

References

Staneck, J. L. & Roberts, G. D. (1974). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226-231.



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Dr. Schumann, Peter
Phone: +49-531/2616-106

MALDI-TOF mass spectrometry of bacteria, RiboPrinting, peptidoglycan analysis, determination of DNA base composition