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Identification and complete phylogenetic analysis of yeasts and related fungiIdentifizierung und komplette phylogenetische Analyse von Hefen und phylogenetisch verwandten filamentösen Pilzen

Identification and complete phylogenetic analysis of yeasts and related fungi

Because identification using morphological characters alone was frequently criticized among mycologists, we do not recommend using this approach alone for isolates with unknown identity.
DSMZ offers standard tests to determine morphological (macro and micro) and physiological (growth rates, media) characters of fungi. These include development of a fungus on standard media, examination of sexual reproduction, and determination of growth characteristics. Depending on a taxonomical group, a few additional tests could be added to comply with the current practice.
Identification of fungal cultures using morphological characters includes subculturing, quick test for purity, examination of sexual reproduction and conidiogenesis, and estimation of radial growth and growth temperatures on selected media. Customers will receive identification report containing the description, identification result (species name and references) and a brief comment on the species.
• Optionally, these tests may be adopted to be used for the standard species description.
• Optionally, a screening of growth responses on various sources or different temperatures can be performed. These can utilize API strips (bioMérieux) or microplates (Biolog, USA), e.g. Foschino et al. (2004), Singh (2009), de Nittis et al. (2010), Meletiadis et al. (2011).
• Tests of other sources and growth conditions are performed on request.
Required material
Customers will need to provide a living culture, agar plate or slant.

 


References

  • de Hoog GS, Guarro J, Gené J, Figueras MJ (2000) Atlas of Clinical Fungi, 2nd edn. Utrecht/Reus: Centraalbureau voor Schimmelcultures/Universitat Rovira i Virgili.
  • de Nittis M, Querol A, Zanoni B, Minati JL, Ambrosoli R (2010) Possible use of Biolog methodology for monitoring yeast presence in alcoholic fermentation for wine-making. J Appl Microbiol. 108, 1199-1206.
  • Foschino R, Gallina S, Andrighetto C, Rossetti L, Galli A (2004) Comparison of cultural methods for the identification and molecular investigation of yeasts from sourdoughs for Italian sweet baked products. FEMS Yeast Res. 4, 609-618.
  • Meletiadis J, Arabatzis M, Bompola M, Tsiveriotis K, Hini S, Petinaki E, Velegraki A, Zerva L (2011) Comparative evaluation of three commercial identification systems using common and rare bloodstream yeast isolates. J Clin Microbiol. 49, 2722-277.
  • Seifert KA, Morgan-Jones G, Gams W, Kendrick B (2011) The genera of Hyphomycetes. CBS Biodiversity Series 9. CBS-KNAW Fungal Biodiversity Centre, Utrecht, Netherlands.
  • Singh MP (2009) Application of Biolog FF MicroPlate for substrate utilization and metabolite profiling of closely related fungi. J Microbiol Methods. 77, 102-108.