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Identification using partial rDNA sequencingIdentifizierung mittels partieller rDNS Sequenzierung

Identification using partial rDNA sequencing

Ribosomal DNA cistron, containing small (18S or SSU) and large (26S/28S or LSU) subunits and the internal transcribed spacer region (rDNA ITS), has been long used as alternative to mitochondrial genes for identification of Fungi (Seifert 2009; Begerow et al. 2010). Despite the progress in developing molecular tools for identification of Fungi, no common DNA marker (barcode) was available. Recently, the utility as DNA-barcodes of five most commonly used gene regions was evaluated (Schoch et al. 2012). As the result, the ITS region was selected to be the first common DNA-barcode for Fungi.
DSMZ offers identification of fungal and yeast cultures using sequencing of the ITS region. Based on these results, a few additional DNA-markers can be applied to perform identification of cryptic species, for instance within common moulds such as Penicillium and Aspergillus.
Identification of fungal cultures with rDNA sequencing includes subculturing, quick test for purity, DNA extraction, PCR, sequencing, and identification report. Customers will receive identification report containing the best match among known species (or available sequences), the identification result (species name and references) and a brief comment on the species.
Required material
A living culture (agar plate or slant) is preferred.


References

  • Begerow D, Nilsson H, Unterseher M, Maier W (2010) Current state and perspectives of fungal DNA barcoding and rapid identification procedures. Appl Microbiol Biotechnol. 87, 99-108.
  • Seifert KA (2009) Progress towards DNA barcoding of fungi. Mol Ecol Resour. 9 s1, 83-89.
  • Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL, Levesque CA, Chen W, Fungal Barcoding Consortium (2012) Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proc Natl Acad Sci USA. 109, 6241-6246.