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MALDI-TOFMALDI-TOF

MALDI-TOF

 

MALDI-TOF

MALDI-TOF MS (matrix assisted laser desorption/ionization time-of-flight mass spectrometry) allows after a simple sample preparation the recording of mass spectra of large biomolecules (mainly ribosomal proteins) which are specific of the microorganism under study. These mass spectrometric fingerprints are used for the reliable identification of bacteria, yeasts and fungi by comparison with reference databases. This method is a promising alternative to microbiological techniques for identification at the species level because it is automated, fast, cost-efficient and suited for highthroughput routine analyses (Schumann & Maier, 2014).

Reference

Schumann, P. & Maier, T. (2014). MALDI-TOF mass spectrometry applied to classification and identification of bacteria. Methods Microbiol  41, 275-306.

 

Photo of Peter  Schumann
Dr. Schumann, Peter
Phone: +49-531/2616-106

MALDI-TOF mass spectrometry of bacteria, RiboPrinting, peptidoglycan analysis, determination of DNA base composition