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DetailsCatalogue
Cell line:
A-549
DSMZ no.:
ACC 107
Species:
human (Homo sapiens)
Cell type:
lung carcinoma
Origin:
established from an explanted lung tumor which was removed from a 58-year-old Caucasian man in 1972; cells were described to induce tumors in athmyic mice and to synthesize lecithin
Reference(s):
14556, 14717
Depositor:
Dr. J. Wehland, GBF, Braunschweig, Germany
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
epithelial cells, growing adherently as monolayer; image; image
Medium:
90% Dulbecco's MEM + 10% h.i. FBS
Subculture:
split confluent cultures 1:5 to 1:10 every 4-7 days using trypsin/EDTA; cells grow easily; seed out at ca. 1-2 x 106 cells/80 cm2 + 10 ml medium
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40 hours
Harvest:
about 20 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule
 
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin - , endothel - , EpCAM -, GFAP - , neurofilament - , vimentin +
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of MDH, NP
Cytogenetics:
human hypotriploid karyotype with 8% polyploidy - 65(59-66)<3n>XXY, -1, -3, -6, +12, -13, -15, -18, -19, -21, -22, +4mar, der(6)t(1;6)(q11;q27), del(11)(q22-23) - presence of large distinctive der(6) marker confirms identity of this cell line
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -, HTLV-I/II -, SMRV -
Supplied as:
Delivery form Prices
Growing culture
  (please inquire for exact delivery time)
 660,- €
Frozen culture 330,- €
DNA isolated from cell line (25 µg)  440,- €
Cell pellet (10-20 x 106 cells) 550,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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