- Cell line:
- VERO-B4
- DSMZ no.:
- ACC 33
- Species:
- monkey - African green monkey (Chlorocebus aethiops (Cercopithecus aethiops))
- Cell type:
- kidney
- Origin:
- established from the kidney of a normal adult African green monkey in Japan in 1962; cells are susceptible to a number of viruses; used in virus replication studies and plaque assays, and as indicator cell line for mycoplasma testing
- Reference(s):
- 15165
- Depositor:
- Dr. H. W. L. Ziegler-Heitbrock, Institute of Immunology, Munich, Germany
- Biosafety level:
- 1
- Permissions and restrictions:
- A, E
- DSMZ Cell Culture Data:
- Morphology:
- adherent-elongated, fibroblast-like cells in monolayers
- Medium:
- 90% RPMI 1640 + 10% h.i. FBS
- Subculture:
- split confluent cultures 1:3 to 1:10 using trypsin/EDTA; seed out at ca. 0.5-1.0 x 106 cells/80 cm2
- Incubation:
- at 37 °C with 5% CO2
- Doubling time:
- ca. 25 hours
- Harvest:
- cell harvest of ca. 10 x 106 cells/80 cm2
- Storage:
- frozen with 70% medium, 20% FBS, 10% DMSO at about 2.5 x 106 cells/ampoule
- DSMZ Scientific Data:
- Mycoplasma:
- negative in DAPI, microbiological culture, RNA hybridization, PCR assays
- Species:
- confirmed as monkey with IEF of AST, LDH, NP and by species PCR
- Cytogenetics:
- simian rearranged flat-moded hypodiploid karyotype with 12% polypoidy - 54(48-61)<2n>X - VERO-B4 markers present
- Viruses:
- ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -
- Supplied as:
Delivery form Prices Frozen culture 300,- € Growing culture
(please inquire for exact delivery time)600,- € DNA isolated from cell line (25 µg) 400,- € Cell pellet (10-20 x 106 cells) 500,- €
see price list- Print data sheet
Stellenausschreibungen:
W2-Professur
„Mikrobielle Genomforschung“
Wiss. Mitarbeiter/-in
iDiv (Postdoc)
25. Mai Tag der offenen Tür
