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DetailsCatalogue
Cell line:
VERO-B4
DSMZ no.:
ACC 33
Species:
monkey - African green monkey (Chlorocebus aethiops (Cercopithecus aethiops))
Cell type:
kidney
Origin:
established from the kidney of a normal adult African green monkey in Japan in 1962; cells are susceptible to a number of viruses; used in virus replication studies and plaque assays, and as indicator cell line for mycoplasma testing
Reference(s):
15165
Depositor:
Dr. H. W. L. Ziegler-Heitbrock, Institute of Immunology, Munich, Germany
Biosafety level:
1
Permissions and restrictions:
A, E
 
DSMZ Cell Culture Data:
Morphology:
adherent-elongated, fibroblast-like cells in monolayers
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
split confluent cultures 1:3 to 1:10 using trypsin/EDTA; seed out at ca. 0.5-1.0 x 106 cells/80 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 25 hours
Harvest:
cell harvest of ca. 10 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO at about 2.5 x 106 cells/ampoule
 
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species:
confirmed as monkey with IEF of AST, LDH, NP and by species PCR
Cytogenetics:
simian rearranged flat-moded hypodiploid karyotype with 12% polypoidy - 54(48-61)<2n>X - VERO-B4 markers present
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -
Supplied as:
Delivery form Prices
Growing culture
  (please inquire for exact delivery time)
 660,- €
Frozen culture 330,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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