JAR
ACC 462
Cell line:
JAR
DSMZ no.:
ACC 462
Species:
human (Homo sapiens)
Cell type:
choriocarcinoma
Origin:
established from the trophoblastic tumor of the placenta of a 24-year-old woman, but the cell line is from the male fetus
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
epithelial-like cells growing adherent in monolayers and in clusters
Medium:
85% RPMI 1640 + 15% h.i. FBS
Subculture:
split culture 1:5 to 1:10 every 3-4 days before reaching confluency using trypsin/EDTA; seed out at ca. 3-4 x 106 cells/80 cm2; confluent cultures tend to form aggregates, that loosely adhere to the culture vessel surface; however, these aggregates are difficult to disperse and may need trypsin treatment after a centrifugation and washing step
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 20-30 hours
Harvest:
cell harvest of ca. 10-15 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, RNA hybridization and continuously in PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded near-triploid karyotype with 27% polyploidy - 60-70<3n>XXY, +1, -2, -5, -7, -8, -9, -13, -13, -14, -15, -17, -19, -21,+12mar, +acf, add(X)(p22), del(1)(p3?p3?), add(1)(q11), der(1)add(1)(p11)add(1)(q4?), inv(3)(p25.2q24), add(3)(q13), add(4)(q?31), del(4)(q2?2), add(9)(p21), add(12)(q24), i(13)(q10)dup(13)(q11q34), add(16)(q24), add(17)(p12), add(18)(p11.2) - sideline with add(2)(p2?), del(8)(q22), add(21)(p11) - resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list