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DetailsCatalogue
Cell line:
BONNA-12
DSMZ no.:
ACC 150
Species:
human (Homo sapiens)
Cell type:
hairy cell leukemia
Origin:
established from the spleen of a 46-year-old man with hairy cell leukemia (HCL) at diagnosis in 1988; cells were described in the literature to be negative for the HCL-associated mutation BRAF V600E
Reference(s):
14672, 15164, 16917
Biosafety level:
1
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1.
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
lymphoblast-like cells growing as single cells and in clumps in suspension; image
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
maintain at 0.5-1.0 x 106 cells/ml; seed out at ca. 0.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-60 hours
Harvest:
saturation density at about 2.0-2.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD10 -, CD19 +, CD20 +, CD34 -, CD37 +, CD80 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of AST, MDH
Cytogenetics:
human hyperdiploid karyotype with 4% polyploidy - 47(45-47)<2n>X, -Y, +12, t(9;10)(p12/13;q12) - supernumerary ring or acf present in most cells
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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