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Analysis of Respiratory Quinones

Respiratory quinones are extracted from freeze dried or fresh cell material using hexane and are further purified by a silica-based solid phase extraction. Purified samples are further analysed by HPLC using a reverse phase column recording absorption spectra.  270 nm for ubiquinones and 326 nm for menaquinones are used for a relative quantification. For complex mixtures, samples are further analysed on an UHPLC-ESI-qTOF system.

Required material: For this method 50 mg freeze dried cells are needed.

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References

  1. Tindall, B.J. (1990a). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst. Appl. Microbiol. 13, 128-130
  2. Tindall, B.J. (1990b). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol. Letts. 66, 199-202