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Analysis of Respiratory Quinones

Respiratory quinones are extracted from freeze dried or fresh cell material using hexane and are further purified by a silica-based solid phase extraction. Purified samples are further analysed by HPLC using a reverse phase column recording absorption spectra.  270 nm for ubiquinones and 326 nm for menaquinones are used for a relative quantification. For complex mixtures, samples are further analysed on an UHPLC-ESI-qTOF system.

Required material: For this method 50 mg freeze dried cells are needed. If the analysis of polar lipids is ordered in parallel we need 200 mg freeze dried cells in total for both methods.

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References

  1. Tindall, B.J. (1990a). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst. Appl. Microbiol. 13, 128-130
  2. Tindall, B.J. (1990b). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol. Letts. 66, 199-202