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Analysis of Respiratory Quinones

Respiratory quinones are extracted from freeze dried or fresh cell material using hexane and are further purified by a silica-based solid phase extraction. Purified samples are analysed by HPLC using a reverse phase column recording absorption spectra (Vieira et. al., 2021).  270 nm for ubiquinones and 326 nm for menaquinones are used for a relative quantification. For complex mixtures, samples are further analysed on an UHPLC-ESI-qTOF system (Schuhmann et. al. 2021).

Required material: For this method 50 mg freeze dried cells (send at ambient temperature) or 500 mg wet biomass (send frozen on dry ice) or suspended in isopropanol (send at ambient temperature) are needed. Alternatively, an actively growing culture (still in exponential growth phase upon arrival, without signs of sporulation or cell lysis) could be send. Subculturing (if required) will be charged separately. If subculturing is requested by the customer, detailed cultivation conditions (media, temperature, oxygen supply, cultivation duration) must be provided.

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  1. Vieira S, Huber KJ, Neumann-Schaal M, Geppert A, Luckner M, Wanner G, Overmann J (2021) Usitatibacter rugosus gen. nov., sp. nov. and Usitatibacter palustris sp. nov., novel members of Usitatibacteraceae fam. Nov. within the order Nitrosomonadales isolated from soil. Int J Syst Evol Microbiol 71:004631.

  2. Schumann P, Kalensee F, Cao J, Criscuolo A, Clermont D, Köhler JM, Meier-Kolthoff JP, Neumann-Schaal M, Tindall BJ, Pukall R (2021) Reclassification of Haloactinobacterium glacieicola as Occultella glacieicola gen. nov., comb. nov., of Haloactinobacterium album as Ruania alba comb. nov., with an emended description of the genus Ruania, recognition that the genus names Haloactinobacterium and Ruania are heterotypic synonyms and description of Occultella aeris sp. nov., a halotolerant isolate from surface soil sampled at an ancient copper smelter. Int J Syst Evol Microbiol 71:004769