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Peptidoglycan of Gram-positive strains from our open collection for reference purposes is prepared as described in Protocol 7 (Schumann, 2011). The yield of peptidoglycan obtained from 4 g wet biomass is usually approximately 10-15 mg but cannot be guaranteed. The minimum amount provided is 5 mg. The production of amounts > 20 mg is beyond the capacities of the DSMZ Service department. Quality evaluation of the peptidoglycan extracts is performed by LC- and GC-MS.

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  1. Schumann, P. (2011). Peptidoglycan structure. Methods Microbiol 38, 101-129.