Analysis of peptidoglycan structure

Analysis of the peptidoglycan structure is a requirement for describing novel genera of Gram-stain positive bacteria. At least the amino acid composition should be provided for novel Gram-stain positive species (Tindall et al., 2010).
Depending on the complexity of the peptidoglycan structure, the elucidation may be time-consuming as different derivatization procedures and analytical techniques (TLC, 2D-TLC, chiral GC, GC/MS) may become necessary. A complete elucidation of hitherto unknown peptidoglycan structures by the DSMZ Identification Service is not always possible.

Required material: 3-4 g wet biomass (centrifugal pellet) sent either in frozen state on dry ice or, alternatively, suspended in isopropanol/water (1:1, v/v) at ambient temperature. Important:Biomass must be harvested before sporulation or autolysis. Lyophilized cells are suboptimal but can also be sent in the amount corresponding to 4 g wet weight. Information on the genus affiliation of the strain under study is helpful for the selection of appropriate techniques and standards.

Dr. Peter Schumann

References

  1. Schumann, P. (2011). Peptidoglycan structure. Methods Microbiol 38, 101-129.
  2. Tindall, B. J., Rosselló-Móra, R., Busse, H.-J., Ludwig, W. & Kämpfer, P. (2010 ). Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 60, 249-266. 
  3. Schleifer, K. H. (1985). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18, 123-156.
  4. Schleifer, K. H. & Kandler, O. (1972). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407-77.