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For the analysis of the microbial diversity of complex samples the V3/V4 region of the 16S rRNA (app. 450 bp) will be amplified and sequenced on an Illumina MiSeq System. A minimum throughput of 200.000 reads per sample is guaranteed.
Sequence data will be compared against the ribosomal 16S database of the NCBI RefSeq Targeted Loci Project. The best hit will be displayed and filtered on a specific taxon level (either on species level or specific customer request). Interactive Chrona charts will be generated, which allow differentiation on species level. The Top 10 of taxa identified will be reported as well as classification of each single read.
Required material: 1 µg of DNA per sample, minimum 10 samples
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- Klindworth A, Pruesse E, Schweer T, Peplies J, Quast C. Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies. Nucleic Acids Res.2013 Jan 7;41(1):e1. doi: 10.1093/nar/gks808. Epub 2012 Aug 28.