Analysis of Mycolic Acids

Analysis on request only.

Dr. Susanne Verbarg

References

  1. Linos, A., Steinbüchel, A., Spröer, C. & Kroppenstedt, R. M. (1999). Gordonia polyisoprenovorans sp. nov., a rubber-degrading actinomycete isolated from an automobile tyre. Int J Syst Bacteriol 49,1785–1791.

Analysis of Whole-Cell Sugars 

Diagnostic sugars in whole-cell hydrolysates (1N H2SO4, 100 °C, 2 hours) are analyzed by TLC on cellulose plates. The result supports the classification and identification of Actinobacteria.

Required material: Either a culture of the strain or 15 mg lyophilized cells.

Dr. Peter Schumann

References

  1. Schumann, P. (2011). Peptidoglycan structure. Methods Microbiol 38, 101-129.
  2. Staneck, J. L. & Roberts, G. D. (1974). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226-231.

Detection of isomers of 2,6-diaminopimelic acid (Dpm) and of 2,6-diamino-3-hydroxypimelic acid (OH-Dpm)

Whole-cell hydrolysates (4N HCl, 100°C, 16 hours) are examined by TLC on cellulose plates for the presence of Dpm isomers or OH-Dpm. The result supports the classification and identification of Actinobacteria.

Required material: Either a culture of the strain or 5 mg lyophilized cells.

Dr. Peter Schumann

References 

  1. Schumann, P. (2011). Peptidoglycan structure. Methods Microbiol 38, 101-129.
  2. Schleifer, K. H. & Kandler, O. (1972). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407-77.
  3. Rhuland, L. E., Work, E., Denman, R. F. & Hoare, D. S. (1955). The behaviour of the isomers of 2,6-diaminopimelic acid on paper chromatograms. J Am Chem Soc 77, 4844-4846.