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DetailsCatalogue
Cell line:
LNCAP
DSMZ no.:
ACC 256
Species:
human (Homo sapiens)
Cell type:
prostate carcinoma
Origin:
established from the left supraclavicular lymph node metastasis from a 50-year-old man with prostate carcinoma in 1977; cells were described in the literature to be androgen-sensitive
Reference(s):
15141, 14557, 14616, 15212
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
adherent fibroblastoid cells growing in aggregates and as single cells; image; image
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
split semi-confluent culture 1:4 once a week using trypsin/EDTA (occasionally cells can be detached by tapping); seed out at ca. 1-2 x 106 cells/25 cm2; after thawing and trypsinization cells may need 1-2 days to become adherent again; due to the strong cell aggregation, it might be difficult to determine an exact cell titer
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 60 hours
Harvest:
cell harvest of about 10-15 x 106 cells/80 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of AST, MDH
Cytogenetics:
human hypotetraploid karyotype with 6% polyploidy - 88(84-93)<4n>XXYY, -2, -4, -6, -6, -13, -13, +2mar, t(1;15)(p22.3;q22.3)x2, del(2)(p16p23), del(10)(q24)x2, der(16)t(6;16)(p22.1;q22)x2 - del(10)(q24) is recurrent in prostate carcinoma - corresponds to published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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