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DetailsCatalogue
Cell line:
P-19
DSMZ no.:
ACC 316
Species:
murine (mouse) (Mus musculus)
Cell type:
embryonal carcinoma
Origin:
established from the teratocarcinoma induced in a C3H/He strain mouse
Reference(s):
14753, 14905
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
epitheloid cells growing as adherent monolayer
Medium:
80-90% alpha-MEM (with ribo- and deoxyribonucleosides) + 10-20% h.i. FBS
Subculture:
split confluent culture 1:10 to 1:20 once or twice a week using trypsin/EDTA; seed out at ca. 0.3-0.4 x 105 cells/cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 2-3 days
Harvest:
cell harvest of max. 0.5 x 106 cells/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species:
originally confirmed as murine with IEF of AST, LDH, MDH, NP and by species PCR and recently as mus musculus by COI DNA barcoding
Cytogenetics:
murine near-diploid karyotype with 16% polyploidy - 40(31-44)<2n> - one centric fusion marker - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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