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Cell line:
DU-4475
DSMZ no.:
ACC 427
Species:
human (Homo sapiens)
Cell type:
breast carcinoma
Origin:
established from the local metastatic cutaneous nodule of an undifferentiated carcinoma of a 62-year-old woman which developped seven years after radical mastectomy of a poorly differentiated ductal carcinoma of the breast; cells were described in the literature to grow readily in athymic nude mice and to produce tumors from which cells could be introduced into culture in vitro; cells reportedly express components of milk fat globules and the BCL1 gene
Reference(s):
14529, 14704
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
large round cells growing singly or in clusters in suspension; image; image
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
split saturated culture 1:2 to 1:3 once a week; seed out at ca. 0.2 x 106 cells/ml; maintain at ca. 0.2 x 106 cells/ml; maximum cell density at ca. 0.5 x 106/ml; due to cluster formation, cells are difficult to count; cultures may contain large amounts of debris and viability may be consistently below 70%
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 80-100 hours
Harvest:
cell harvest of ca. 0.3-0.4 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 -, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of AST, MDH, NP (loss of LDH isoenzymes)
Cytogenetics:
human flat-moded near-tetraploid karyotype with 12% polyploidy - 88-93<4n>XXXX, +1, +1, -5, -6, +9, -10, -10, +15, +15, -16, -16, +22, +4mar, i(1q)x2, ?add(1)(p35-36)x2, ?i(5p)x2, add(6)(p11), add(6)(p1?), del(6)(q25), add(9)(q35), del(11)(q24)x2, add(15)(p11)x2, add(17)(p1?)x2, del(21)(q22.2)x2 - sideline with -20, -20, +del(7)(p11) - gain of 1q and loss of 6q typical in breast carcinoma - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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