EVSA-T
ACC 433
Cell line:
EVSA-T
DSMZ no.:
ACC 433
Species:
human (Homo sapiens)
Cell type:
breast carcinoma
Origin:
established from the malignant ascitic effusion from a woman with metastatic breast carcinoma; described originally as being estrogen receptor-negative and progesterone receptor-positive (the latter not confirmed by DSMZ);
RNA sequence data are available (see Ref 40906 and RNA-Seq)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
adherent epitheloid cells growing in monolayers
Medium:
90% MEM (with Earle's salts) + 10% h.i. FBS + 2 mM L-glutamine
Subculture:
seed out at ca. 2-5 x 106 cells/80 cm2; split semi-confluent culture 1:3 every 3-4 days using trypsin/EDTA, dissociate cells with a pipette (10 ml) during trypsination; after thawing and in the first week, viability and adherence may be low (about 50%)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
cell harvest of about 20-30 x 106 per 80cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypotriploid karyotype with 4% polyploidy - 62(55-64)<3n>XXX, +1, -2, -5, -5, +6, -8, -9, -10, -13, -13, -14, -15, -16, -17, -18, -20, -22, +6-8mar, add(X)(p11), der(X)t(X;9)(q21;q11), add(1)(p1?1), der(1)del(1)(p36)dup(1)(q22q32), del(1)(q11), der(1;4)(q10;q10)del(4)(q1?13q?23), der(2)t(2;5)(p11;q13)add(2)(q3?5), der(3)t(3;5)(p14;q11), del(4)(q22), del(6)(q25q27), del(6)(p11), add(6)(p23), del(7)(p14), add(8)(q24), ider(8)(q10)ins(8;?)dup(8)(?q21q24)add(8)(q24), add(11)(p15), add(12)(p13), del(12)(p13), dup(13)(q21q33), add(21)(p11), add(21)(p11), large submetacentric der(8) hsr marker present
Molec. Genetics:
Western blot : ER, PR, AR negative while positive for HER2
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV +, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list