Go to ContentTo Startpage
DetailsCatalogue
Cell line:
CAKI-2
DSMZ no.:
ACC 54
Species:
human (Homo sapiens)
Cell type:
kidney carcinoma
Origin:
established from the primary renal carcinoma of a 69-year-old man
Reference(s):
14537, 14538
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
epithelial-like cells, growing as adherent monolayer
Medium:
90% McCoy's 5A + 10% h.i. FBS
Subculture:
change medium twice every week, split confluent cultures 1:2 to 1:3 weekly using trypsin/EDTA, upon thawing grow cells immediately in 80 cm2; seed out at ca. 2 x 106 cells/80 cm2 in 10 ml medium
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
cell harvest of ca. 1 x 106 cells/25 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin +
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of AST, MDH, NP
Cytogenetics:
human flat-moded pentaploid/hexaploid karyotype with 12% polyploidy - 130(118-132)<6n>XXX, -Y, -Y, -Y, inc, add(1)(p22), i(8q), i(?5q)x2, del(6)(q23), t(14;15)(p10;q10), der(19)t(1;19)(q24;q13), dminx1, large iso der(5q)x2, and submeta marker der(?)t(?;2)(?;q11)x2 - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
Print data sheet