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DetailsCatalogue
Cell line:
C2C12
DSMZ no.:
ACC 565
Species:
murine (mouse) (Mus musculus)
Cell type:
myoblasts
Origin:
subclone of a mouse myoblast cell line established from mouse strain C31+; C2C12 cells are described to differentiate rapidly forming myotubes; reportedly treatment with bone morphogenic protein 2 induces differentiation into the osteoblast lineage
Reference(s):
14393, 14651
Depositor:
Dr. D. Fleckenstein, TU Braunschweig, Germany
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
polymorphic cells growing adherently in monolayers
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
split semi-confluent culture 1:10 to 1:50 every 2-4 days using trypsin/EDTA (do not allow cultures to become confluent) ; seed out 1-5 x 105 cells in 80 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 20 hours
Harvest:
cell harvest of ca. 10-15 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO at about 0.5 x 106 cells/ampoule
 
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Species:
confirmed as mouse by PCR species analysis
Cytogenetics:
murine flat moded near-tetraploid karyotype
Viruses:
PCR: SMRV -
Supplied as:
Delivery form Prices
Growing culture
  (please inquire for exact delivery time)
 660,- €
Frozen culture 330,- €
DNA isolated from cell line (25 µg)  440,- €
Cell pellet (10-20 x 106 cells) 550,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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