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DetailsCatalogue
Cell line:
HELA
DSMZ no.:
ACC 57
Species:
human (Homo sapiens)
Cell type:
cervix carcinoma
Origin:
established from the epitheloid cervix carcinoma of a 31-year-old black woman in 1951; later diagnosis changed to adenocarcinoma; first aneuploid, continuously cultured human cell line
Reference(s):
14926, 14555, 18012, 18013
Biosafety level:
1
Risk assessment:
The cell line is infected with papilloma virus type 18 (HPV-18). The cells contain several copies as proviruses integrated into the eukaryotic genome. The integrated virus genomes are incomplete and exhibit 2-3 kb deletions of the E2-L2 region. An activation and transmission of the HPV-18 during handling of the cell line is improbable. The cell line is categorized biosafety level 1.
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
epithelial-like cells growing in monolayers; image; image
Medium:
90% MEM (with Earle's salts) + 10% h.i. FBS + 2 mM L-glutamine + non-essential amino acids (cells also grow well in 90-95% RPMI 1640 + 5-10% h.i. FBS)
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:4 to 1:6 every 3-5 days using trypsin/EDTA; cells reach confluence quickly
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-48 hours
Harvest:
cell harvest of ca. 10-15 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM -, GFAP -, HMB-45 -, neurofilament -, vimentin +
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of G6PD, MDH, NP
Cytogenetics:
human hypertriploid/hypotetraploid karyotype with 15% polyploidy - 82(77-84)<4n>XXX, -X, -2, -3, -4, +5, +5, +5, +5, -7, -8, -11, -13, -14, -15, -16, -17, -18, -19, -21, -22, +mar - der(1)t(1;3)(p11;q11), add(2)(q37), del(3)(q13), der(3)t(3;5)(q11;q11), i(5p)x4, del(6)(q15), der(9)t(1;9)(p11;p11), add(12)(q23), i(15q), der(19)t(13;19)(q22;p13)x1-2, add(21)(p11), del(?22)(q12)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV +, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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