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DetailsCatalogue
Cell line:
BHK-21
DSMZ no.:
ACC 61
Species:
Syrian hamster (golden hamster) (Mesocricetus auratus)
Cell type:
kidney
Origin:
BHK-21 is a subclone (clone 13) of the parental line established from the kidneys of five unsexed, one-day-old hamsters in 1961; cells can be used for virus replication studies
Reference(s):
14727, 14726
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
fibroblastoid adherent monolayer; image; image
Medium:
90% MEM (with Earle's salts) + 10% h.i. FBS + 1x non-essential amino acids
Subculture:
split confluent culture 1:2 to 1:3 every 2-4 days using trypsin/EDTA; seed out at ca. 0.2 x 105 cells/cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 32-50 hours
Harvest:
about 10-15 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Fingerprint:
unique DNA profile with (gtg)5 multilocus probe
Species:
originally confirmed as Syrian (Golden) Hamster by species PCR and recently as Mesocricetus auratus origin confirmed by COI DNA barcoding
Cytogenetics:
near-diploid golden (Syrian) hamster karyotype with 21% tetraploidy - 40-45, XY - hyperdiploid sideline present at 19% - resembles published description
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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