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DetailsCatalogue
Cell line:
MOLM-6
DSMZ no.:
ACC 611
Species:
human (Homo sapiens)
Cell type:
chronic myeloid leukemia in blast crisis
Origin:
established from the peripheral blood of a 44-year-old man with Philadelphia chromosome-positive chronic myeloid leukemia (CML) in blast crisis in 1992
Reference(s):
15023
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
single, round cells in suspension; some cells may be adherent; image; image
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
maintain at 1-1.5 x 106 cells/ml; optimal split ratio of 1:2 to 1:3 twice a week; seed out at ca. 0.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48-72 hours
Harvest:
cell harvest of ca. 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 +, HLA-DR +; image
Fingerprint:
fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile
Species:
confirmed as human by cytogenetics
Cytogenetics:
human flat moded near triploid karyotype; 62-77<3n>XY/XXY, +6, +6, -14, -15, -16, -17, -18, +22, +22, +2mar, t(9;22)(q34;q11), i(11q), der(22)t(9;22)(q34;q11)x3; add(7)(p11) present in sdl; cells typically carry 4 copies of der(22)t(9;22) / Ph effecting BCR-ABL1 rearrangement
Molec. Genetics:
expression of fusion gene BCR-ABL1 e13-a2 (b2-a2) confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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