FKH-1

human (Homo sapiens)

acute myelocytic leukemia

established in 1993 from the peripheral blood of a 61-year-old man with Philadelphia chromosome negative chronic myeloid leukemia (CML) with trilineage myelodysplasia at refractory leukemic transformation into acute myeloid leukemia (AML M4); carries t(6;9)(p23;q34) leading to DEK-NUP214 (DEK-CAN) fusion gene; contrary to what was originally described the cells do not respond to G-CSF

1

A

Transfer to third parties needs to be negotiated with the depositor.

mostly single, round cells in suspension; image; image; image

80% RPMI 1640 + 20% h.i. FBS

seed out at ca. 1.0 x 10⁶ cells/ml in 24- or 12-well plates; optimal split ratio of 1:2 or less every 3-4 days; cultures may contain a high amount of cell debris; the accumulation of cell debris can be prevented by using new culture vessels every second week

at 37 °C with 5% CO₂

ca. 60-100 hours

maximum density at ca. 3 x 10⁶ cells/ml

frozen with 70% medium, 20% FBS, 10% DMSO

negative in microbiological culture, PCR assays

CD3 -, CD4 +, CD11b +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, HLA-DR +; image

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

human hypodiploid karyotype with 4% polyploidy; 45(43-46)<2n>XY, -7, t(6;9)(p23;q34); carries t(6;9) effecting rearrangement of DEK with NUP214 (CAN); closely resembles published karyotype

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Delivery form		Prices
Frozen culture		450,- €
Growing culture   (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

Print data sheet