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DetailsCatalogue
Cell line:
P30-OHKUBO
DSMZ no.:
ACC 727
Species:
human (Homo sapiens)
Cell type:
B cell precursor acute lymphoblastic leukemia
Origin:
established from the bone marrow of an 11-year-old girl with acute lymphoblastic leukemia (ALL FAB L2) after 3rd relapse following bone marrow transplantation in 1980
Reference(s):
16668, 14744
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
single cells growing in suspension; image; image
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.1-2.0 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30 hours
Harvest:
maximum density at ca. 3 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 -, CD38 +, cyCD79a +, CD80 -, CD138 +, HLA-DR +, cyIgG -, cyIgM +, cykappa -, cylambda +; image
Fingerprint:
fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile
Cytogenetics:
human flat-moded hypodiploid karyotype; 45(42-46)<2n>X, -X, der(2)t(2;4)(p22;q31), der(3)t(3;21)(q28;q22.2), der(4)t(4;9)(q24;q32), der(6)t(6;14)(q25;q24), der(8)t(4;8)(q24;q24)t(4;13)(q31;q31), der(9)del(9)(p21p23)inv(9)(p21q13)t(2;9)(p22;q13), der(9)t(8;9)(q23;p21), del(12)(q23q24), der(12)t(6;12)(q25;p13), del(14)(q31); related to published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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