N4TG3
ACC 101
Cell line:
N4TG3
DSMZ no.:
ACC 101
Species:
murine (mouse) (Mus musculus)
Cell type:
neuroblastoma
Origin:
6-thioguanine resistant mutant of cell line N4 that was established from a mouse of strain A/J with neuroblastoma C1300; cells were described in the literature to form increased levels of cAMP in response to prostaglandin E1 which is inhibited by opiate receptors; cells are aneuploid (modal chromosome number of 86)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% DMEM + 10% h.i. FBS + 100 µM 6-thioguanine
Subculture:
split saturated culture 1:2 to 1:4 every 2-4 days (cells grow slightly adherent, but can be detached with trypsin)
Incubation:
at 37 °C with 10% CO2
Harvest:
about 10-20 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Viruses:
ELISA: reverse transcriptase positive
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list