F9
ACC 112
Cell line:
F9
DSMZ no.:
ACC 112
Species:
murine (mouse) (Mus musculus)
Cell type:
embryonal carcinoma
Origin:
initiated from a testicular teratocarcinoma of a strain 129 mouse
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
adherent cells with undifferentiated morphology growing in clumps
Medium:
90% Dulbecco's MEM (4.5 g/L glucose) + 10% h.i. FBS
Subculture:
presoak flasks with 0.1% gelatin at 4 °C for 2 hours, wash 3x with PBS; split confluent culture 1:10 to 1:20 every 2-4 days using trypsin/EDTA; seed out at ca. 2 x 106 cells/80 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 24 hours
Harvest:
about 70 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine near-diploid karyotype with 6% tetraploidy - 38(37-40)<2n> - 1-2 centric fusion markers present
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list