GIRARDI HEART C2 - Discontinued
ACC 116
Cell line:
GIRARDI HEART C2 - Discontinued
DSMZ no.:
ACC 116
Species:
human (Homo sapiens)
Cell type:
cervix carcinoma (cell line HELA)
Origin:
Caution: Isoenzyme and STR analyses demonstrated that Girardi Heart C2 is genetically identical to HELA. Girardi Heart C2 must not be used as a model for human heart cells.
Reference(s):
Biosafety level:
1
Risk assessment:
The cell line is infected with papilloma virus type 18 (HPV-18). The cells contain several copies as proviruses integrated into the eukaryotic genome. The integrated virus genomes are incomplete and exhibit 2-3 kb deletions of the E2-L2 region. An activation and transmission of the HPV-18 during handling of the cell line is improbable. The cell line is categorized biosafety level 1.
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
epithelial cells growing as monolayers
Medium:
45% Medium 199 (with Hank's salts) and 45% RPMI 1640 + 10% h.i. FBS
Subculture:
split confluent culture 1:3 to 1:6 every 3-4 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Harvest:
about 10 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis resulted in the STR profile of cell line HELA.
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV +, HTLV-1/2 -, MLV -, SMRV -
Supplied as: