MB-04
ACC 122
Cell line:
MB-04
DSMZ no.:
ACC 122
Species:
insect - cabbage moth (Mamestra brassicae) (Mamestra brassicae)
Cell type:
larval hemocyte
Origin:
established from the larvae of the butterfly Mamestra brassicae (family Noctuidae, subfamily Noctuinae) (cabbage moth; Kohleule)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% Grace's Insect (Modified) Medium + 10% h.i. FBS
Subculture:
cells are difficult to culture! split cultures 1:2 every 2-3 days (cells grow partly adherent, but trypsin is not necessary); maintain at room temperature (about 20 °C) with CO2 exposure ; confluent cultures can be stored at 4 °C for up to one week without loss of viability; after thawing cells might have a lag phase of up to 4 weeks
Incubation:
at 20 °C (room temperature) without CO2
Harvest:
about 5-7 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mamestra brassicae species.
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list