RVH-421
ACC 127
Cell line:
RVH-421
DSMZ no.:
ACC 127
Species:
human (Homo sapiens)
Cell type:
melanoma
Origin:
established from the tumor biopsy of an intracranial secondary melanoma in a 28-year-old man in 1979; it was described that increasing the tyrosine concentration enhances synthesis of melanin
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
adherent, epithelial-like cells growing in monolayers; image
Medium:
90% RPMI 1640 (or Leibovitz's L-15) + 10% h.i. FBS;
incubation: at 37°C and 5% CO2, with gas exchange for RPMI 1640 but without gas exchange for Leibovitz' L15 (no filter lid, lid of flask closed)
Subculture:
seed out 0.5-2 x 106 cells/175 cm2; split confluent culture up to 1:10 one to two times per week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2 for RPMI 1640, but no gas exchange for Leibovitz's L-15
Doubling time:
ca. 50-60 hours
Harvest:
about 15 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-triploid karyotype with 30% polyploidy - 69(60-70)<3n>XXY, -10, +13, -15, +16, -20, +mar, del(6)(q15), add(8)(q24), del(9)(p22), der(9)t(7;9)(q12;q34)x2, add(11)(q24-25), ins(12;abr)(?q13), der(13)(t(6;13)(p13;q33)x2-3, add(19)(p13)
Molec. Genetics:
Genomic sequencing and/or ARMS assay show that the cell line carries the BRAF V600E mutation (mu/wt), but no NRAS Q61 mutation
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list