established from the peripheral blood of a 19-year-old man with acute lymphoblastic leukemia (ALL) in relapse in 1976. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence
and RNA-Seq)
single, small, round cells in suspension; image; image; image
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/ml; maintain at 1.0-2.0 x 106 cells/ml; split ratio of 1:2 to 1:3 every 3 days; minimum density of 0.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 36 hours
Harvest:
maximal density of 4-6 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays
